David specializes in cellular biology, protein biochemistry, structural biology, and immunotherapy/immuno-oncology.
2018 (expected), Yale University, Ph.D., Pharmacology
2015, Yale University, M.Phil, Pharmacology
2014, Yale University, M.S., Pharmacology
2010, Boston College, B.S., Biochemistry
David Puleo, a technology specialist and PhD candidate in the Pharmacology Department at Yale University, combines extensive research and leadership experience to provide a unique approach to biotechnology and the law. Prior to attending Yale, David graduated from Boston College with a B.S. in Biochemistry. His interest in drug discovery prompted him to work in the Center for Proteomic Chemistry at Novartis Institutes for BioMedical Research in Cambridge, MA for two years. During his time at Yale, David has collaborated with Gilead Sciences on an early stage drug discovery project to develop novel targeted therapies for immune-related diseases. Outside of the lab, he is also the Vice President of the Yale Biomedical Careers Committee, an organization that facilitates career exploration and professional development for graduate students and post-doctoral fellows.
Invited as Short Talk Speaker at the 2017 Keystone Symposia on ‘Kinases: Next-Generation Insights and Approaches’ in Breckenridge, CO
Awarded Phi Beta Kappa, Boston College
The Boston College Excellence in Chemistry Award, Boston College
Scholar of the College in Advanced Biochemistry Research Award, Boston College
Awarded The William H. Prusoff Award in Pharmacology, Yale University
Puleo, D.E., Kucera, K., Hammarén, H.M., Ungureanu, D., Newton, A.S., Silvennoinen, O., Jorgensen, W.L. & Schlessinger, J. Identification and Characterization of JAK2 Pseudokinase Domain Small Molecule Binders. ACS Med. Chem. Lett. 8, 618–621 (2017).
Newton, A.S.*, Deiana, L.*, Puleo, D.E., Cisneros, J.A., Cutrona, K.J., Schlessinger, J. & Jorgensen, W.L. JAK2 JH2 Fluorescence Polarization Assay and Crystal Structures for Complexes with Three Small Molecules. ACS Med. Chem. Lett. 8, 614–617 (2017). *Co-first author
Touré, B.B. et al. Toward the Validation of Maternal Embryonic Leucine Zipper Kinase: Discovery, Optimization of Highly Potent and Selective Inhibitors, and Preliminary Biology Insight. J. Med. Chem. 59, 4711-4723 (2016).
Frey, K.M., Puleo, D.E., Spasov, K.A., Bollini, M., Jorgensen, W.L. & Anderson, K.S. Structure-Based Evaluation of Non-nucleoside Inhibitors with Improved Potency and Solubility That Target HIV Reverse Transcriptase Variants. J. Med. Chem. 58, 2737-2745 (2015).
Harris, K.V., Cockrell, G.M., Puleo, D.E. & Kantrowitz, E.R. Crystallographic Snapshots of the Complete Catalytic Cycle of the Unregulated Aspartate Transcarbamoylase from Bacillus subtilis. J. Mol. Biol. 411, 190-200 (2011).